SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Summary and Recommendations of the International Workshop on Aflatoxin Contamination of Groundnut

D. McDonald.

Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.

Ano: 1987

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3144

Coordinated Research on Groundnut Rosette Virus Disease

Palavras-chave: Mycotoxins; Groundnuts; ELISA; Genotypes; Sampling; Detoxification; Chromatography; Toxins; Fungi; Immunological techniques.

Ano: 1988

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4056

Summary Proceedings of the Second Coordinators' Meeting on Peanut Stripe Virus

Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.

Ano: 1988

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4054

Bud Necrosis:Bud Necrosis: A Disease of Groundnut Caused by Tomato Spotted Wilt Virus

Palavras-chave: Viruses; Groundnuts; Genotypes; Yield losses; ELISA; Peptides; Quarantine; Legumes; Germplasm; HPLC.

Ano: 1989

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4070

Contribution à l'étude du système neuroendocrine de la moule Mytilus edulis (Mollusca: Bivalvia)

D.V.R..

Palavras-chave: Necrosis; ELISA; Groundnuts; Sucrose; Amines; Insecticides; Cytoplasmic organelles; Poultry equipment; Viruses; Antibiotics.

Ano: 1990

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4665

ArchiMer

Kellner-cousin, Kristell.

Dans le cadre de ce travail, l'étude du système neuroendocrine de la moule Mytilus edulis a été envisagée sous plusieurs aspects qui définissent les quatre grandes parties de la thèse. Dans une première partie est décrit le travail de préparation et de caractérisation d'anticorps monoclonaux (AcMc) spécifiques de ganglions cérébroïdes (GC) de Mytilus edulis. Le développement de techniques de préparation d'AcMc dirigés contre des antigènes non purifiés a en effet permis de produire un ensemble de réactifs de type AcMc dirigés contre différents antigènes issus d'un broyat de GC de moule. La spécificité de ces AcMc a été étudiée en microscopie optique puis électronique selon des techniques immunocytochimiques (immunopéroxydase indirecte et immunogold). La...

Tipo: Text

Palavras-chave: Mollusque; Bivalve; Mytilus edulis; Cellules neurosécrétrices; Système nerveux; Système immunitaire; Controle de la croissance; Insuline; Anticorps monoclonaux; Immunocytocbimie; ELISA; PCR; Banque d'ADNc.

Ano: 1993

URL: http://archimer.ifremer.fr/doc/00109/22019/19642.pdf

SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: II. ASSOCIATION BETWEEN PREVALENCE AND LEVEL OF ELISA RESPONSE

Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles.

A herd of cattle (Holstein-Zebu crosses) was screened every two months by ELISA during a period of two years for IgG antibodies against 19 infectious disease agents. Two hundred and ninety five sera were collected from 157 young animals (0-4 months of age), 1037 sera from 292 developing animals (4-36 months of age) and 1468 sera from 259 producing animals (> 36 months of age). The results indicate that the difference in ELISA between positive and negative tests is associated with the overall prevalence of positive tests. When the prevalence of positive tests is low the difference between positive and negative tests is greater than when the prevalence is intermediate or high. This means that ELISA, presumably other serological tests for IgG antibodies,...

MARKOV CHAIN MODELING OF ENDEMIC CATTLE DISEASES IN THE TROPICS OF MEXICO

Palavras-chave: Serological screening; Cattie diseases; ELISA.

Ano: 1993

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200014

Rojas,José Alfonso Barajas; Riemann,Hans; Franti,Charles.

Endemic diseases measured by prevalence of IgG antibodies by ELISA all showed remarkably stable overall prevalences over a study period of 3 years. This however does not mean that incidence is low or absent. Observed transition from the state of being positive for a disease to the state of being negative and vice versa suggested fairly high incidence rates (and rates of waning antibodies) for all 19 disease agents tested for. In spite of this dynamic situation Markov Chain simulations indicate that a steady prevalence is reached in a fairly short time. Markov Chain simulations may also be useful in elucidating seasonal changes in prevalence.

SEROLOGICAL SCREENING FOR INFECTOUS CATTLE DISEASES: III. CHOICE OF SENTINEL ANIMALS

Palavras-chave: Prevalence; Markov Chain; México; ELISA.

Ano: 1993

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300014

Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles.

A herd of Cattle (Holstein-Zebu crosses) was screened bimonthly for two years by ELISA for IgG antibodies to infectious disease agents. The herd was composed of 3 age groups: Young animals ( < 4 months of age), developing animals (4-36 months of age) and producing animals ( > 36 months of age). Ali young animals received colostrum within two hours after birth. The overall consistency of ELISA results, i. e agreement between two consecutive tests was about the same for the three age groups and was lowest for infections of intermediate prevalence (40-50%). The interpretation of changes in seroprevalence is most straight forward for young animals. Young animals positive at their first test reflect the immune status of their mothers while young...

Working together on groundnut virus diseases

Palavras-chave: ELISA; Seroconversion; Young; Sentinels; Infectious diseases.

Ano: 1993

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200015

Working Together on Groundnut Virus Diseases

D.V.R..

Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.

Ano: 1994

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4178

ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT

D.V.R.( editor).

Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.

Ano: 1994

URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3121

Aradaib,Imadeldin Elamin; Abbas,Babiker; Osburn,Bennie; Bushara,Hamid Omer; Taylor,Martin.

Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks...

Diagnostic Methods for the Detection of Cryptosporidium parvum Oocysts in Faeces

Palavras-chave: ELISA; Schistosoma bovis; Vaccine.

Ano: 1994

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020

OAK

Webster, K. A.; Green, J. A.; Dawson, C.; Giles, M.; Catchpole, J..

Palavras-chave: Cryptosporidium parvum; Diagnosis; Immunofluorescence; ELISA; Coproscopical.

Ano: 1996

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/253

Estudo comparativo entre as provas de ELISA e imunofluorescência indireta (IFI) para detectar anticorpos contra Babesia bovis

Martins,João Ricardo; Corrêa,Bartolomeu Lima; Ceresér,Victor Hermes.

Comparou-se o desempenho de um kit de ELISA para detectar anticorpos contra Babesia bovis, em hemoparasita bovino, com o teste de Imunofluorescência Indireta (IFI) usualmente empregado em rotina sorológica. Sobre 97 amostras de soros oriundas de uma região livre de carrapatos e hematozoários, o teste de ELISA demonstrou uma especificidade de 98.9% contra 97.9% do teste de IFI. Ambos os testes apresentaram uma sensibilidade de 100% quando utilizados sobre 22 amostras de soro de bovinos experimentalmente infectados com B. bovis. Os resultados obtidos sobre 1560 amostras colhidas à campo, mostraram uma concordância de 90,1% (1406/1560) entre amostras positivas e negativas, enquanto que 4,2% (66) foram positivas para IFI e negativas para ELISA e 5,6% (88)...

Recherche et caractérisation d'immunoglobulines dans les oeufs du loup (Dicentrarchus labrax)

Palavras-chave: Sorologia; Babesia bovis; ELISA; IFI.

Ano: 1996

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000100021

ArchiMer

Pepin, Jean-francois; Breuil, Gilles; Rigobert, P; Romestand, Bernard.

The occurrence of maternal immunity transfer in Sea bass (Dicentrarchus labrax) was investigated using ELISA and Western blot technics. In order to determine the possible presence of immunoglobulin (Ig), egg proteins obtained from fertilized eggs were analyzed. After several extraction protocol assays (precipitation, chromatography), monoclonal and polyclonal antibody probes specific to Sea bass Ig allowed to confirm the presence of an immunoglobulin-like protein in eggs with very low level. Biochemical characterization was carried out using SDS-PAGE. Results indicated that egg Ig had a molecular weight of 206 kDa. They were smaller than seric IgM (800 kDa). The heavy (H) and light (L) chains of the egg Ig and of the seric IgM had similar molecular weight,...

Tipo: Text

Palavras-chave: Immunoglobuline; Transfert immunité maternelle; ELISA; Œuf; Poisson; Immunoglobulin; Maternal immunity transfer; ELISA; Eggs; Fish.

Ano: 1997

URL: http://archimer.ifremer.fr/doc/00272/38275/36458.pdf

Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil

BJMBR

Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A..

We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....

Utilização de um teste de ELISA polivalente para detecção de anticorpos contra Actinobacillus pleuropneumoniae (App)

Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005

Arq. Bras. Med. Vet. Zootec.

Kich,J.D.; Piffer,I.A.; Guidoni,A.L.; Barcellos,D.E.S.N.; Klein,C.S..

Um teste de ELISA polivalente baseado em lipopolissacarídeos de cadeia longa (LPS - CL) de Actinobacillus pleuropneumoniae (App) sorotipos 3, 5 e 7 foi avaliado testando-se amostras do soro de leitões e matrizes provenientes de 10 rebanhos positivos e de 10 rebanhos negativos. Foram classificados como positivos aqueles rebanhos com isolamento prévio do App sorotipos 3, 5 ou 7 e rebanhos negativos aqueles submetidos ao controle veterinário, sem notificação de sintomas clínicos, sem lesões de pleuropneumonia suína e sem isolamento do agente. Todos os rebanhos positivos apresentaram sorologia positiva e as matrizes apresentaram maior número de soroconversores (P<0,05) do que os leitões. Entre os rebanhos negativos quatro apresentaram sorologia negativa,...

Determination of serum isometamidium levels in sheep and goats under field conditions using isometamidium-ELISA

Palavras-chave: Suínos; Actinobacillus pleuropneumoniae; Pleuropneumonia; ELISA.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500002

OAK

Wesongah, J. O.; Murilla, G. A.; Kibugu, J. K.; Jones, T. W..

Twenty eight sheep and 28 goats were treated with isometamidium chloride (Samorin®, hone Merieux, Lyon, France) at a dose of 1 mg/kg body weight (bw) by intramuscular injection. All the animals were grazed in a tsetse-infested area. They were monitored for anaemia, body weight, anti-trypanosome antibodies and serum isometamidium concentration using the isometamidium enzyme-linked immunosorbent assay (ELISA) for more than 80 days after treatment. Serum isometamidium levels were higher in goats than in sheep throughout the experimental period. Isometamidium was still detectable in sheep and goats for up to 77 and 98 days, respectively, alter treatment (detection limit=0.1 ng/ml). The isometamidium elimination half-lives in sheep and goats were approximately...

Palavras-chave: Isometamidium chloride; ELISA; Sheep; Goats; Trypanosomosis.

Ano: 2000

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/132

Anticorpos Para o Vírus da Anemia das Galinhas (CAV) em Matrizes de Corte no Brasil

Rev. Bras. Ciênc. Avic.

Brentano,L; Silva,BG; Sayd,S; Flores,SW.

Apesar do diagnóstico da anemia infecciosa e mais evidências recentes de infecção em campo (Brentano & Ottati, 1999), não havia até hoje dados disponíveis da prevalência do vírus da anemia das galinhas (CAV) no Brasil. Uma vez que o conhecimento da prevalência do vírus e da distribuição de anticorpos nos lotes de matrizes é essencial para o delineamento de estratégias de controle da doença, realizamos um levantamento em 5 Estados com produção comercial intensiva de linhas de corte, abrangendo lotes de aves com diferentes idades. De 2.355 soros testados por ELISA, 89% foram positivos, indicando a alta prevalência do CAV no Brasil, em todos os Estados testados. A análise do soro de aves das 6 até as 70 semanas de idade demonstrou que lotes de 6 a 18...

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals

Palavras-chave: Anemia infecciosa das galinhas; Vírus da anemia das galinhas; Anticorpos; ELISA.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2000000200006

Arq. Bras. Med. Vet. Zootec.

Carvalho,R.; Passos,L.M.F.; Gouvea,A.M.G.; Resende,M.; Martins,A.S.; Franco,G.C..

An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.